Preparation of Golgi Membranes
نویسندگان
چکیده
Anisotropy of anionic sites on plasma membranes has been implicated in contact phenomena at cell surfaces (16, 17). Recent evidence indicates that charge anisotropy may be important among intracellular membranes also, since distribution of anionic sites has been related to structural organization of mitochondrial membranes (8). The use of cationic ferritin has been introduced by Danon et al. (2) as a marker to demonstrate anionic sites on membrane surfaces by electron microscopy. This visual probe has been applied to study anionic sites on cell surfaces (2, 7) and on mitochondrial membranes (8). In the present study, cationic ferritin has been used to demonstrate anionic sites on the surface of the Golgi complex in relation to the structural organization of this membrane system.
منابع مشابه
Analytical study of microsomes and isolated subcellular membranes from rat liver VIII. Subfractionation of preparations enriched with plasma membranes, outer mitochondrial membranes, or Golgi complex membranes
Preparations enriched with plasmalemmal, outer mitochondrial, or Golgi complex membranes from rat liver were subfractionated by isopycnic centrifugation, without or after treatment with digitonin, to establish the subcellular distribution of a variety of enzymes. The typical plasmalemmal enzymes 5'-nucleotidase, alkaline phosphodiesterase I, and alkaline phosphatase were markedly shifted by dig...
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